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Table 1 List of primers used in this study

From: Biocatalytic synthesis of flavones and hydroxyl-small molecules by recombinant Escherichia coli cells expressing the cyanobacterial CYP110E1 gene

  primer sequence species
CYP284A1 Ana1361F 5'-TAC CAT ATG ATG CTC CAA TAC ATT ACT GCT CTC-3'  
Ana1361R 5'-TAC GAA TTC ATT TCT CAA CCG AAA GCG CAC T-3'  
CYP110A1 Ana1450F 5'-TAC CAT ATG ATG TTG ACT CAA TTA CCA AAT CC-3'  
Ana1450R 5'-TAC GAA TTC GTT GAA AAT CTT GCT ACT TTG CT-3'  
CYP110B1 Ana3746F 5'-TAC CAT ATG ATG CAC CTA CCA AAA GG-3'  
Ana3746R 5'-CAC GAA TTC ACT TAC AGT AGT TGT TTC TAG-3'  
    Nostoc sp. PCC 7120
CYP110C1 Ana4686F 5'-CAC CAT ATG ATG AAA TAT CAA ATA CAG AGA CC-3'  
Ana4686R 5'-TAC AAG CTT TGC GTT GAA TGT TGT TGA G-3'  
CYP110D1 Ana4766F 5'-TAC CAT ATG ATG ACA GTC ACT CAA AAC C-3'  
Ana4766R 5'-CAC AAG CTT CGA ATT ACG CAT TCT TTT ATT AG-3'  
CYP110E1 Ana4833F 5'-TAC CAT ATG ATG AAA CTT CCA GAT AGT C-3'  
  Ana4833R 5'-TAC GAA TTC TAC TTC TAC AGG GTT TTT G-3'  
CYP110C3 Ana1981F 5'-TAC CAT ATG ATG AAG TAT CAA ATA AAG AGA C-3'  
Ana1981R 5'-TAC GAA TTC TGT TGT GAA TGT TGT TGA G-3'  
CYP110E6 Ana2103F 5'-TAC CAT ATG ATG AAA CTT CCA GAT AGT CC-3'  
Ana2103R 5'-TAC GAA TTC TAC TTC TAC AGG GCT TTT GA-3'  
    A. variabilis ATCC 29413
CYP110A2 Ana3921F 5'-TAC CAT ATG ATG TTG ACT CAA TTA CCA AA-3'  
Ana3921R 5'-CAC GAA TTC ATT AAA AAT CTT GTT ACT TTG CT-3'  
CYP284A3 Ana4063F 5'-TAC CAT ATG ATG CTC CAA TAC GTT ACT GCT C-3'  
  Ana4063R 5'-TAC GAA TTC ATT TCT CAA CCG AAA GCG CAC-3'  
CYP110F1 Nos0984F 5'-TAC CAT ATG ATG AAA ATA CTT GAT AGT CTA AC-3'  
Nos0984R 5'-TAC AAG CTT AGT AGA AAG TAT TGT TTG TCT TT-3'  
CYP110B2 Nos0985F 5'-TAC CAT ATG ATG AAA TTA CCA AAA GGC C-3'  
Nos0985R 5'-TAT GAA TTC AAC AGT GGC TGT CTG-3'  
CYP197B1 Nos2212F 5'-TAC CAT ATG ATG GTT GCC GAT GTA TT-3'  
  5'-CAC GAA TTC TTT AGA AGT GTC TAA TGC AA-3'  
CYP284A2 Nos2399F 5'-TAC CAT ATG ATG TTC CAA CAG ATT GCT GC-3'  
Nos2399R 5'-TAC GAA TTC ACG AGC GAT ATT GTC AGA GT-3'  
CYP120B1 Nos2686F 5'-TAC CAT ATG ATG AAA ACT AAT CAA ATT CCT-3'  
Nos2686R 5'-TAT GAA TTC CCG AGG TTG AAA TCT-3'  
    N. punctiforme PCC 73102
CYP110D2 Nos3640F 5'-CTA C CA TAT GAT GAA AAG TCG TAA CAA TAA AA-3'  
Nos3640R 5'-TAT GAA TTC AAC TAG GGC TGG C-3'  
CYP110C2 Nos6291F 5'-TAC CAT ATG ATG CAA CTA CCT AAT ATT CT-3'  
Nos6291R 5'-TAT GAA TTC GGA TAG GGG TGT AG-3'  
CYP110E2 Nos7017F 5'-GCA GCA TAT GAT GTC TTT ACT TAA ACT G-3'  
Nos7017R 5'-TCA C GA ATT CAA CTG AAC TAG AGC T-3'  
CYP227A1 Nos7684F 5'-ATA C CA TAT GAT GAC ACT TAA AGA TAA AG-3'  
Nos7684R 5'-TAT GAA TTC CAG TCG TTG AGC AA-3'  
CYP120C1 Nos8095F 5'-TAC CAT ATG ATG CAG CAG TTA AAA TCC G-3'  
  Nos8095R 5'-TAC GAA TTC ACT ATC CAA GGG ATG CTT T-3  
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Microbial Cell Factories

ISSN: 1475-2859

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