Skip to main content
Figure 5 | Microbial Cell Factories

Figure 5

From: Physiological heterogeneities in microbial populations and implications for physical stress tolerance

Figure 5

Effect of freeze-thaw stress on GFP signal. Mean GFP level of the entire population prior to freeze-thaw stress (circles) and mean GFP level of PI negative cells (triangles) and PI positive cells (squares) after freeze-thaw stress plotted against point of harvest during aerobic batch cultivation. The GFP signal was significantly decreased in cells that have been permeabilised by the stress exposure, hence making PI staining redundant to estimate number of cells with intact membranes.

Back to article page