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Table 1 List of transcripts whose blockage leads to growth stoppage

From: An inverse metabolic engineering approach for the design of an improved host platform for over-expression of recombinant proteins in Escherichia coli

Transcript name

Gene name

Size (bp)

Known or putative function of the gene product

Orientation

Essential/ Non essential

GN35 (T7)

-

221

Ketoacid-binding protein

Reverse

Non-essential

CN38 (T7)

cysN

567

Adenosine 5’ phosphosulphate kinase sulfate adenylyltransferase subunit 1

Same direction

Non-essential

BN43 (T7)

kdpD

568

Sensory histidine kinase

Reverse

Non-essential

GN11 (T7)

ribB

403

3,4 dihydroxy-2-butanone-4-phosphate synthase

Reverse

Non-essential

EN34 (T7)

mfd

587

Transcription repair coupling factor

Same direction

Non-essential

MN45 (T7)

aroC

190

Chorismate synthase

Reverse

Non-essential

G3 (T7)

yijE

379

Predicted permease

Reverse

Non-essential

A50 (T7)

gpt

141

Xanthine-guanine phosphoribosyl transferase

Reverse

Non-essential

F4 (T7)

yjgF

122

Ketoacid binding protein

Reverse

Non-essential

H36 (T7)

rpsU

422

Acid resistant protein

Same direction

Non-essential

E48 (T7)

yidE

350

Predicted transporter

Reverse

Non-essential

G15 (T7)

ygcQ

 

Predicted electron transfer flavoprotein, NAD/FAD binding

Same direction

Non-essential

G18 (T7)

galT

375

Galactose −1 phosphate uridylyltransferase

Reverse

Non-essential

G20 (T7)

fdnG

720

Formate dehydrogenase- N,alpha subunit,nitrate inducible

Reverse

Non-essential

H7 (T7)

ynaA

876

Conserved hypothetical protein: Rac prophage

Reverse

Non-essential

H30 (T7)

yrfD

769

 

Reverse

Non-essential

I7 (T7)

wzyE

770

Predicted Wzy protein involved in ECA polysacharide

Same direction

Non-essential

L27 (ara)

-

639

Conserved hypothetical protein

Reverse

Non-essential

A17 (ara)

bioF

414

Predicted methyltransferase, enzyme of biotin synthesis

Reverse

Non-essential

A29 (ara)

kdpF

289

2-octaprenylphenol hydroxylase enzyme

Reverse

Non-essential

F50 (ara)

-

313

iro p300

Reverse

Non-essential