Figure 3
From: Recombinant production of self-assembling β-structured peptides using SUMO as a fusion partner
Average absorbance traces of cleaved SUMO_P 11 -N when purified using RP-HPLC on a C18 column. Absorbance measurements were made at 280 nm and 220 nm with the fraction collector programmed to collect peaks at 220 nm. The positions of the peaks subsequently identified to contain the peptide and SUMO protein are indicated by arrows. A) P11-4 purification with the sharp peak at 6–7 minutes corresponding to P11-4 and the broader peak between 11 and 20 minutes corresponding to SUMO. B) P11-13 purification. C) P11-14 (K) purification.