Skip to main content

Table 5 Fermentation profiles of haploid yeast strain YGLY13979 in comparison with its homozygous diploid strain YGLY19895

From: Generation of diploid Pichia pastoris strains by mating and their application for recombinant protein production

Strain

Ploidy

Supernatant HPLC Titer (mg/L)

Broth Titer (mg/L)

WCW (g/L)

Induction Time (hr)

YGLY13979

Haploid

1048

780

255

96

YGLY19895

Diploid

920

730

206

96

Strain

Ploidy

Man5%

G0%

G1%

G2%

Hybrid %

Complex %

YGLY13979

Haploid

13.3

60.1

16.8

3.8

6.0

80.7

YGLY19895

Diploid

15.6

59.1

15.1

2.5

7.7

76.7

  1. *Data represents the average measurement values from triplicate fermentation runs.
  2. Man5: Man5GlcNac2;
  3. G0: GlcNAc2Man3GlcNAc2;
  4. G1: Gal1GlcNAc2Man3GlcNAc2
  5. G2: Gal2GlcNAc2Man3GlcNAc2;
  6. Hybrid: GlcNAc1Man5GlcNAc2 + Gal1GlcNAc1Man5GlcNAc2.
  7. Complex: G0 + G1 + G2.