Table 3 Primers used to confirm the existence of anti-HER2 light and heavy chains in diploid genome
Reaction | Primer Name | Primer Sequence (5' to 3') | Primer Description | Product |
|---|---|---|---|---|
PCR-Hc | MT3 | GGTTCCAATTGACAAGCTTTTGATTTTAACG | AOX1p_Forword | 970 bp |
MT126 | GTCCTCGTGAGAAACGTC | Anti_HER2_Hc_Reverse | ||
PCR-Lc | MT3 | GGTTCCAATTGACAAGCTTTTGATTTTAACG | AOX1p_Forword | 580 bp |
MT132 | CTCTCTTGGGTAGAAGTTGTTCAACAA | Anti_HER2_Lc_Reverse |