Table 2 Mating efficiency of OCH1 wild-type and glycoengineered P. pastoris strains
Parental Haploid Strain | Glycan Structure | Mating Condition | Diploid Selection Markers | Estimated Maximum Mating Efficiency |
|---|---|---|---|---|
NRRL-Y11430 | OCH1 wild-type | 3 days, 25°C | ARS, NAT | 10-2 |
NRRL-Y11430 | OCH1 wild-type | 3 days, 25°C | Arg1, His1 | 10-2 |
NRRL-Y11430 | OCH1 wild-type | 3 days, 25°C | Arg3, His1 | 10-2 |
NRRL-Y11430 | OCH1 wild-type | 3 days, 25°C | Arg3, His4 | 10-2 |
YGLY2-3 | och1 Δ | 5 days, 25°C | ARS, NAT | 10-3 |
YGLY2-3 | och1 Δ | 5 days, 25°C | Zeocin, ARS | 10-3 |
YGLY2-3 | och1 Δ | 5 days, 25°C | Arg3, His1 | 10-3 |
YGLY8292 | GS2.0 (Man5) | 5 days, 25°C | Zeocin, ARS | 10-3 |
YGLY8292 | GS2.0 (Man5) | 5 days, 25°C | Zeocin, NAT | 10-3 |
YGLY8292 | GS2.0 (Man5) | 5 days, 25°C | ARS, NAT | 10-3 |
YGLY8292 | GS2.0 (Man5) | 5 days, 25°C | Arg3, His1 | 10-3 |
YGLY8316 | GS5.0 (G2) | 5 days, 25°C | Zeocin, ARS | 2.5 × 10-4 |
YGLY8316 | GS5.0 (G2) | 5 days, 25°C | Zeocin, NAT | 2.5 × 10-4 |
YGLY8316 | GS5.0 (G2) | 5 days, 25°C | ARS, NAT | 2.5 × 10-4 |
YGLY8316 | GS5.0 (G2) | 5 days, 25°C | Arg3, Ura5 | 10-5 * |