Generation of diploid Pichia pastoris strains by mating and their application for recombinant protein production

Chen, Ming-Tang; Lin, Song; Shandil, Ishaan; Andrews, Dewan; Stadheim, Terrance A; Choi, Byung-Kwon

doi:10.1186/1475-2859-11-91

Table 1 Yeast strains used in this study

From: Generation of diploid Pichia pastoris strains by mating and their application for recombinant protein production

Strain	Ploidy	Parent	Plasmid	Glycan Structure	Genotype
NRRL-Y11430	haploid	N/A	N/A	wild-type	OCH1 wild-type N-glycan P. pastoris
YGLY2-3	haploid	N/A	N/A	och1Δ	och1- N-glycan P. pastoris
YGLY8292	haploid	N/A	N/A	GS2.0 (Man5)	och1- glycoengineered GS2.0 starting strain*
YGLY8316	haploid	N/A	N/A	GS5.0 (G2)	och1- glycoengineered GS5.0 starting strain*
YGLY27429	haploid	NRRL-Y11430	pGLY10969	wild-type	URA6::AOX1p-anti-HER2-Lc, TEFp-mCherry, Ars
YGLY27430	haploid	NRRL-Y11430	pGLY10970	wild-type	URA6::AOX1p-anti-HER2-Hc, TEFp-GFP, Nat
YGLY27431	diploid	YGLY27429/YGLY27430	N/A	wild-type	URA6::AOX1p-anti-HER2-Lc, TEFp-mCherry, Ars
YGLY27431	diploid	YGLY27429/YGLY27430	N/A	wild-type	URA6::AOX1p-anti-HER2-Hc, TEFp-GFP, Nat
YGLY27432	haploid	NRRL-Y11430	pGLY10969 pGLY10970	wild-type	URA6::AOX1p-anti-HER2-Lc, TEFp-mCherry, Ars
YGLY27432	haploid	NRRL-Y11430	pGLY10969 pGLY10970	wild-type	URA6::AOX1p-anti-HER2-Hc, TEFp-GFP, Nat
YGLY27433	haploid	YGLY8292	pGLY10969	GS2.0 (Man5)	URA6::AOX1p-anti-HER2-Lc, TEFp-mCherry, Ars
YGLY27434	haploid	YGLY8292	pGLY10970	GS2.0 (Man5)	URA6::AOX1p-anti-HER2-Hc, TEFp-GFP, Nat
YGLY27435	diploid	YGLY27433/YGLY27434	N/A	GS2.0 (Man5)	URA6::AOX1p-anti-HER2-Lc, TEFp-mCherry, Ars
YGLY27435	diploid	YGLY27433/YGLY27434	N/A	GS2.0 (Man5)	URA6::AOX1p-anti-HER2-Hc, TEFp-GFP, Nat
YGLY27436	haploid	YGLY8292	pGLY10969 pGLY10970	GS2.0 (Man5)	URA6::AOX1p-anti-HER2-Lc, TEFp-mCherry, Ars
YGLY27436	haploid	YGLY8292	pGLY10969 pGLY10970	GS2.0 (Man5)	URA6::AOX1p-anti-HER2-Hc, TEFp-GFP, Nat
YGLY13979	haploid	YGLY8316	pGLY6830	GS5.0 (G2)	TRP2::AOX1p-anti-HER2-Lc, AOX1p-anti-HER2-Hc, Zeocin
YGLY19313	haploid	YGLY13979	N/A	GS5.0 (G2)	TRP2::AOX1p-anti-HER2-Lc, AOX1p-anti-HER2-Hc, Zeocin, ura5-
YGLY19853	haploid	YGLY19313	pGLY24	GS5.0 (G2)	TRP2::AOX1p-anti-HER2-Lc, AOX1p-anti-HER2-Hc, Zeocin, arg3-
YGLY19895	diploid	YGLY19313/YGLY19853	N/A	GS5.0 (G2)	TRP2::AOX1p-anti-HER2-Lc, AOX1p-anti-HER2-Hc, Zeocin
YGLY19895	diploid	YGLY19313/YGLY19853	N/A	GS5.0 (G2)	TRP2::AOX1p-anti-HER2-Lc, AOX1p-anti-HER2-Hc, Zeocin

*Detailed strain genotype is described in Materials and Methods.
Man5: Man₅GlcNac₂;
G2: Gal₂GlcNAc₂Man₃GlcNAc₂;

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ISSN: 1475-2859

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