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Figure 6 | Microbial Cell Factories

Figure 6

From: Generation of diploid Pichia pastoris strains by mating and their application for recombinant protein production

Figure 6

Characterization of diploid P. pastoris -derived antibody. (a) SDS-PAGE showing the quality of an anti-HER2 antibody produced in diploid P. pastoris strains in comparison with anti-HER2 material produced in haploid P. pastoris strains and their comparison with trastuzumab produced from CHO cells. (b) LC-MS mass spectrometry of anti-HER2 antibodies after release of N-glycan. For a, and b, anti-HER2 material produced in yeast strains was purified by affinity capture using protein A beads.

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Microbial Cell Factories

ISSN: 1475-2859

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