Figure 5
Evaluation of diploid strain stability using flow cytometry analysis. (a) Green and red fluorescence intensities of parental haploid yeast strain YGLY27430 (expressing only GFP). (b) Green and red fluorescence intensities of parental haploid yeast strain YGLY27429 (expressing only RFP). (c) Green and red fluorescence intensities of diploid yeast strain YGLY27431 (expressing both GFP and RFP). (d) Time course of green and red fluorescence intensities of diploid yeast strain YGLY27431 in shake-flask, methanol induction media. Fluorescence intensities were measured at 24-hour time intervals. (e) Fluorescence intensities of diploid yeast strain YGLY27431 after 72 hours cultivation in nitrogen-deprived sporulation media. For a, b, c, d and e, all yeast strains contained OCH1 wild-type N-glycan. Similar flow cytometry profiles were also observed in several different GS2.0 strain lineages YGLY27433, YGLY27434, and YGLY27435 (data not shown).