Figure 4

Diploid strain stability analysis. (a) Diploid strain stability showing total diploid population percentage in shake flasks followed by a methanol induction phase over 72 hour induction time course. (b) Time-dependent diploid stability in nitrogen-deprived sporulation media. For a, and b, percentage of diploid population was determined by the number of colonies growing on a diploid selection plate in comparison with the number of colonies growing in a non-selective plate. Symbols represent the average measurement values from triplicate experiments. As controls, parental haploid strains carrying either ARS or NAT selection marker did not form any colonies on selection plates containing both arsenic and nourseothricin (data not shown).