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Table 1 Macro parameters in MTP, Shake flask and Bioreactor

From: Robust, small-scale cultivation platform for Streptomyces coelicolor

Β 

Un-optimized

MTP1

Optimized

MTP2

SF2

Bioreactor3

ΞΌ max (h -1 )

0.03 Β± 0.008

0.11 Β± 0.01

0.11 Β± 0.01

0.10 Β± 0.005

Final Biomass (g/L)

2.0 Β± 0.13

4.0 Β± 0.13

3.7 Β± 1.3

4.1 Β± 0.1

UDP (mg/L)

6.4 Β± 1.6

80 Β± 4.4

28 Β± 15.5

65 Β± 3.3

ACT (mg/L)

18.5.0 Β± 7.5

520 Β± 17.0

267 Β± 23.6

500 Β± 17.7

  1. Biomass, UDP and ACT concentration indicated in the table corresponds to 120 hrs.
  2. 1Data based on 4 biological replicates each in triplicate. Un-optimized MTPs contained a medium with MES buffer and no glass beads.
  3. 2Data based on 3 biological replicates each in triplicate. Optimized MTPs and Shake flasks (SF) contained a medium with 100 mM MOPS buffer to maintain constant pH and 3 mm glass beads.
  4. 3Data based on three cultivations.
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Microbial Cell Factories

ISSN: 1475-2859

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