Figure 6

Infectivity of Sup35-NM IBs. Induction of different [PSI+] strains upon transformation of a [psi-] yeast strain with the soluble (S), insoluble (I) fractions of E. coli cells expressing Sup35-NM protein at 18 and 37°C or purified Sup35-NM IBs. After PEG transformation with the indicated material, yeast cells were recovered on SD-URA and randomly selected colonies were spotted onto ¼ YPD plates to identify [PSI⁺] converted colonies. [psi-] and [PSI+] columns correspond to the parental negative and positive control strains. Transformation with the bacterial material induced pink (weak) and to white (strong) [PSI+] phenotypes. Representative images of spots corresponding to distinct strains are shown for each transformed material (see Additional file 1: Table S1 for quantitative data).