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Figure 2 | Microbial Cell Factories

Figure 2

From: A structurally informed autotransporter platform for efficient heterologous protein secretion and display

Figure 2

Secretion of ESAT6 upon fusion to a truncated Hbp passenger. (A) Secretion of Hbp constructs compared to the empty vector control (EV), analyzed by SDS-PAGE and Coomassie staining as described in the legend to Figure 1. (B) Analysis of the samples described under A by immunoblotting using ESAT6 specific antibodies. The amount of ESAT6 recovered from the respective cultures was quantified and displayed at the bottom of the panel as relative amounts (%) compared to Hbp(Δd1)-ESAT6. Molecular mass (kDa) markers are indicated at the left side of the panels. Cleaved passenger (>) is indicated.

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Microbial Cell Factories

ISSN: 1475-2859

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