From: Engineering of daidzein 3’-hydroxylase P450 enzyme into catalytically self-sufficient cytochrome P450
Kinetic parameters
3’-DH-CamA/B
3’-ASDH
K m [μM]
21.8 ± 3.6
20.5 ± 5.1
k cat
15.0 ± 2.1
342.3 ± 53.6
[min-1]
k cat /K m
0.7 ± 0.4
16.8 ± 4.6
[μM-1 min-1]
Coupling efficiency (%)
< 55
< 72