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Table 1 Comparison of kinetic parameters of P450s with natural redox partners and P450s fused to aself-sufficient mimic system

From: Engineering of daidzein 3’-hydroxylase P450 enzyme into catalytically self-sufficient cytochrome P450

Kinetic parameters

3’-DH-CamA/B

3’-ASDH

K m [μM]

21.8 ± 3.6

20.5 ± 5.1

k cat

15.0 ± 2.1

342.3 ± 53.6

[min-1]

k cat /K m

0.7 ± 0.4

16.8 ± 4.6

[μM-1min-1]

Coupling efficiency (%)

< 55

< 72