Table 1 Bacterial strains and plasmids used in this study
Strain | Relevant characteristics | Reference |
|---|---|---|
E. coli | ||
DH10B | Cloning host | Invitrogen™ |
BL21 (DE3) | Expression host | Invitrogen™ |
Bifidobacterium | ||
B. bifidum S17 | intestinal isolate from a breast-fed infant | [23] |
B. bifidum S16 | intestinal isolate from a breast-fed infant | [23] |
B. bifidum NCC189 | NCC | |
B. bifidum NCIMB41171 | NCIMB | |
B. breve S27 | intestinal isolate from a breast-fed infant | This study |
B. breve DSM20213 | type strain, intestinal isolate from an infant | DSMZ |
B. longum/infantis E18 | intestinal isolate from an adult | [23] |
B. longum/infantis NCC200 | NCC | |
B. longum NCC2705 | type strain | NCC |
B. longum DSM20219 | type strain, intestinal isolate from an adult | DSMZ |
B. adolescentis NCC251 | type strain | NCC |
B. adolescentis DSM20083 | type strain, intestinal isolate from an adult | DSMZ |
B. lactis NCC362 | type strain | NCC |
B. lactis L15 | isolate from a commercial dairy product | This study |
B. animalis MB254 | type strain | [23] |
Plasmids | ||
pMDY23 | E. coli-Bifidobacterium shuttle vector harbouring the gusA reporter gene, Spcr | [33] |
pMGS_PBAD_bopA His6 | pMGS containing bopA under control of arabinose-inducible PBAD promoter | This study |
pMGS_Pgap_bopA His6 | pMGS containing bopA under control of constitutive Pgap promoter | This study |