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Table 2 AmyK38 production in dltB mutants with or without an up-shift of external pH

From: High external pH enables more efficient secretion of alkaline α-amylase AmyK38 by Bacillus subtilis

Strain Plasmid Na2CO3a α-amylase activity(kU l-1)b
168 pHYK38 - 2.9 ± 0.3
168ΔdltB pHYK38 - 7.0 ± 0.3
MGB874 pHYK38 - 0.10 ± 0.01
874ΔdltB pHYK38 - 0.27 ± 0.02
168 pHYK38 + 14.3 ± 0.2
168ΔdltB pHYK38 + 14.8 ± 0.4
MGB874 pHYK38 + 20.8 ± 0.8
874ΔdltB pHYK38 + 20.1 ± 0.2
  1. aB. subtilis derivative strains were grown in 2xL-Mal medium at 30°C. At the transition phase (12 h), an Na2CO3 solution was added to the growth medium at a final concentration of 0.9% (w/v). -, No addition; +, Addition.
  2. bα-amylase activity was determined after 72 h of cultivation.