Table 1 Yield and purification grade throughout processing of recombinant enzyme
| Â | Activity (Units) | Protein (mg) | Yield (%) | Ae (U/mg) | Purification factor |
|---|---|---|---|---|---|
Culture liquid | 9539 | 140 | 100 | 68 | 1.0 |
Ultrafiltrate | 9839 | 89 | 103 | 111 | 1.6 |
HIC-octyl sepharose and removal of Triton X-100 | 5624 | 9 | 59 | 625 | 9.2 |