A new stoichiometric miniaturization strategy for screening of industrial microbial strains: application to cellulase hyper-producing Trichoderma reesei strains

Table 2 Conditions used for the stoichiometric miniaturization strategy

		bioreactor protocol	fed-flask protocol	miniaturization fold
Working volume (mL)		750	50	/15
Biomass concentration (g L^-1)		17	7.5	/2.2
Feed composition	lactose (g L^-1)	250	50	/5
Feed composition	NH₃ (mM)	(separate¹)	160
Feed rate (mL h^-1)		2	0.3	/6.6
specific lactose feed rate (g g^-1 h^-1)		0.04	0.04	constant

Experimental conditions for reference fed-batch production in bioreactor, and miniaturization fold applied for the design of the fed-flask protocol (at constant specific lactose feed rate). ¹For bioreactor protocol, NH₃ was added on demand by pH control system, whereas it was stoichiometrically mixed with the feed for fed-flask protocol (see Figure 3).

ISSN: 1475-2859