Host cell and expression engineering for development of an E. coli ketoreductase catalyst: Enhancement of formate dehydrogenase activity for regeneration of NADH

Table 3 E. coli expression strains addressed in this study

E. coli host	Plasmid	Enzyme	Antibiotic	Abbreviation
JM109	pBTac1	Cb FDH	Amp¹	JM109_FDH [8]
BL21 (DE3)	pRSF-1b	Cb FDH	Kan²	BL21_FDH
Rosetta 2 (DE3)	pRSF-1b	Cb FDH	Cam³, Kan	Rosetta_FDH
BL21 star (DE3)	pRSF-1b	Cb FDH	Kan	Star_FDH
BL21 (DE3)	pETDuet-1	Ct XR, Cb FDH	Amp	BL21_XR_FDH
BL21 (DE3)	pETDuet-1 pRSF-1b	Ct XR, Cb FDH; Cb FDH	Amp, Kan	BL21_XR_2FDH
Rosetta 2 (DE3)	pETDuet-1	Ct XR, Cb FDH	Cam, Amp	Rosetta_XR_FDH
Rosetta 2 (DE3)	pETDuet-1 pRSF-1b	Ct XR, Cb FDH; Cb FDH	Cam, Amp, Kan	Rosetta_XR_2FDH
BL21 star (DE3)	pETDuet-1 pRSF-1b	Ct XR, Cb FDH; Cb FDH	Amp, Kan	Star_XR_2FDH

ISSN: 1475-2859