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Table 3 E. coli expression strains addressed in this study

From: Host cell and expression engineering for development of an E. coli ketoreductase catalyst: Enhancement of formate dehydrogenase activity for regeneration of NADH

E. coli host Plasmid Enzyme Antibiotic Abbreviation
JM109 pBTac1 Cb FDH Amp1 JM109_FDH [8]
BL21 (DE3) pRSF-1b Cb FDH Kan2 BL21_FDH
Rosetta 2 (DE3) pRSF-1b Cb FDH Cam3, Kan Rosetta_FDH
BL21 star (DE3) pRSF-1b Cb FDH Kan Star_FDH
BL21 (DE3) pETDuet-1 Ct XR, Cb FDH Amp BL21_XR_FDH
BL21 (DE3) pETDuet-1 pRSF-1b Ct XR, Cb FDH; Cb FDH Amp, Kan BL21_XR_2FDH
Rosetta 2 (DE3) pETDuet-1 Ct XR, Cb FDH Cam, Amp Rosetta_XR_FDH
Rosetta 2 (DE3) pETDuet-1 pRSF-1b Ct XR, Cb FDH; Cb FDH Cam, Amp, Kan Rosetta_XR_2FDH
BL21 star (DE3) pETDuet-1 pRSF-1b Ct XR, Cb FDH; Cb FDH Amp, Kan Star_XR_2FDH
  1. 1 Ampicillin 115 mg/L; 2 kanamycin 50 mg/L; 3 chloramphenicol 34 mg/L.