Table 4 Plasmid used in this study
From: Production of 2,3-butanediol in Saccharomyces cerevisiae by in silico aided metabolic engineering
Plasmid | Description | Reference |
|---|---|---|
pFA6a-KanMX6 | E. coli kanr gene under the control of Ashbya gossypii TEF promoter-driven | [45] |
pCNKanMX6 | loxP flanking KanMX6 at SalI/BamHI and SacI/EcoRV sites | This study |
pTKURA3 | loxP flanking S. cerevisiae URA3 gene | This study |
p423GPD | 2 μ, HIS3, P GPD1 | [46] |
p423TEF | 2 μ, HIS3, P TEF1 | [46] |
p423GPD-BDH1 | S. cerevisiae BDH1 gene under the control of GPD1 promoter in p423GPD | This study |
p423TEF-BDH1 | S. cerevisiae BDH1 gene under the control of TEF1 promoter in p423GPD | This study |
p423GPD-E.a.budC | E. aerogenes KCTC 2190 budC gene under the control of GPD1 promoter in p423GPD | This study |
pESC-URA | 2 μ, URA3, P GAL10 P GAL1 | Stratagene |
pESC-LEU | 2 μ, LEU2, P GAL10 P GAL1 | Stratagene |
pESC-LEU-Cre | P1 bacteriophage Cre recombinase under the control of GAL10 promoter | This study |
pCTP-URA | 2 μ, URA3, P TEF1 P PGK1 | This study |
pCTP-URA::TEF1p-alsS + PGK1p-budA | Bacillus subtilis alsS gene under the control of TEF1 promoter and E. aerogenes KCTC 2190 budA gene under the control of PGK1 promoter in pCTP-URA | This study |