Skip to main content

Table 2 S. cerevisiae strains used in this study

From: Production of 2,3-butanediol in Saccharomyces cerevisiae by in silico aided metabolic engineering

Strain

Genotype

Reference

BY4742

MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0

EUROSCARF

B2C-a1

BY4742 Δadh1::loxP

This study

B2C-a1a3

BY4742 Δadh1::loxP Δadh3::loxP

This study

B2C-a1a5

BY4742 Δadh1::loxP Δadh5::loxP

This study

B2C-a1a3a5

BY4742 Δadh1::loxP Δadh5::loxP Δadh3::loxP

This study

B2C-a1a6

BY4742 Δadh1::loxP Δald6::loxP

This study

B2C-a1a3a6

BY4742 Δadh1::loxP Δald6::loxP Δadh3::loxP

This study

B2C-a1a5a6

BY4742 Δadh1::loxP Δald6::loxP Δadh5::loxP

This study

B2C-a1a6g2

BY4742 Δadh1::loxP Δald6::loxP Δgpd2::loxP

This study

B2C-a1a3a5a6g2

BY4742 Δadh1::loxP Δald6::loxP Δgpd2::loxP Δadh3::loxP Δadh5::loxP

This study

B2C-SB

BY4742 p423GPD-BDH1

This study

B2C-ABC

BY4742 p423GPD-budC pCTP-URA::TEF1p-alsS + PGK1p-budA

This study

B2C-a1a5-SB

BY4742 Δadh1::loxP Δadh5::loxP p423TEF-BDH1

This study

B2C-a1a5-ABC

BY4742 Δadh1::loxP Δadh5::loxP p423GPD-budC pCTP-URA::TEF1p-alsS + PGK1p-budA

This study

B2C-a1a6-SB

BY4742 Δadh1::loxP Δald6::loxP p423GPD-BDH1

This study

B2C-a1a6-ABC

BY4742 Δadh1::loxP Δald6::loxP p423GPD-budC pCTP-URA::TEF1p-alsS + PGK1p-budA

This study

  1. *Genotype is described in the order of the corresponding genetic manipulations.