Production of 2,3-butanediol in Saccharomyces cerevisiae by in silico aided metabolic engineering

Table 1 Deletion strategies suggested by OptKnock algorithm for the production of 2,3-butanediol

ID	Target reaction for knockouts*	Corresponding gene(s) for knockouts	Maximum specific growth rate (1/hr)	R,R-2,3-Butanediol yield (g/g glucose)
WT	-	-	0.288	0
A	1. ALCD2ir:	ADH1, ADH4, ADH5	0.231	0.313
	2. ALCD2irm	ADH3
	3. ALCD2x	SFA1
B	1. ALCD2ir	ADH1, ADH4, ADH5	0.190	0.362
	2. ALCD2x	SFA1
	3. GTPCI	FOL2
	4. MDH	MDH2
C	1. GLUDyi	GDH1, GDH3	0.266	0.151
	2. NADK	UTR1, UTR2, POS5
	3. NDPK3	YNK1
	4. PDHm	PDB1, PDA1, LAT1, LPD1, PDX1
D	1. ASPTA	AAT2	0.264	0.151
	2. CYTK1	-
	3. GLUDyi	GDH1, GDH3
	4. PDHm	PDB1, PDA1, LAT1, LPD1, PDX1
	5. TMDPP	PNP1

* Additional descriptions are provided in Additional file 1.
The maximum specific growth rate and R,R-2,3-butanediol yield were calculated based on 10 mmol·gDCW^-1.hr^-1 of glucose uptake. WT stands for the complete network of iMM904 model.

ISSN: 1475-2859