Table 2 Primers used in this study
Gene | Primer name | Sequence (5'→ 3') |
|---|---|---|
BTE | BTE-NdeF | GGAATTCCATATG GCCACCACCTCTTTAG |
BTE-NotR | ATAAGAATGCGGCCGC TTACACCCTCGGTTCTGCG | |
BTE-NcoF | CTAGCCATGG CCACCACCTCTTTAGC | |
BTE-XhoR | CACCTCGAG TTACACCCTCGGTTCTGCG | |
T7min-BamF | CAGGGATCC TAATACGACTCACTATAGG | |
‘tesA | tesA-NcoF | CATGCCATGG CGGACACGTTATTGATTCTG |
tesA-EcoR | CAGGAATTC TTATGAGTCATGATTTACTAAAG | |
fadD | fadD-BglF | CTCAGATCT CATGAAGAAGGTTTGGCTTAACC |
fadD-XhoR | CACCTCGAG TTATCAGGCTTTATTGTCCACTTTG | |
yngl | yngl-Eco32F | CACGATATC GATGGCTGAACTCATCCATTCC |
yngl-XhoR | CACCTCGAG TCATTGACATGATGATAAGTTG | |
FAA2 | FAA2-NdeF | GGAATTCCATATG GCCGCTCCAGATTATGCAC |
FAA2-Eco32R | CACGATATC TTACTAAAGCTTTTCTGTCTTGACTAG | |
FAR | FAR-NcoF | CATGCCATGG AGGAAATGGGCAGC |
FAR-BamR | CGGGATCC TTAGTTAAGAACGTGCTCCAC | |
CER4 | CER4-NcoF | CATGCCATGG GCATGTCGACAGAAATGGAGGTCG |
CER4-BamR | CGGGATCC TTAGAAGACATACTTAAGCAGCCC | |
acr1 | acr1-NcoF | CATGCCATGG TGAACAAAAAACTTGAAGC |
acr1-BamR | CGGGATCC TTACCAGTGTTCGCCTG |