Parameters (Growth in) | C1 | C4 | C6 | C7 |
---|---|---|---|---|
Liquid medium after 7 d | Single suspended cells | Clump formation | ||
Nutrient agar | very good: white-yellow, flat colonies, 2–4 mm Ø | very good: white-yellow, flat colonies with dark raised center, 2–4 mm Ø | very good: white-yellow, flat colonies up to 3 mm Ø | |
Tryptic-Soy agar | very weak: orange-yellow, round colonies upto 1.5 mm Ø | no growth | very good: orange-yellow, round, little slimy colonies with raised yellow center and colorless edge upto 4 mm Ø | very good: orange-yellow, round, little slimy colonies with raised yellow center and colorless edge upto 2.5 mm Ø |
Yeast-Malt agar | very good: white colonies with flattened center upto 2 mm Ø | very good: white colonies with flattened center upto 3 mm Ø | very good: yellowish-white, very slimy colonies with a dark center upto 4 mm Ø | |
Yeast-Mannitol agar with 0.025% Congo red | very good: slimy colonies, pink color > confirm Agrobacterium sp. as according to Bergey’s manual Rhizobium sp. forms white colonies on this medium | |||
1/10 Tryptic-Soy broth + 100 μg/ml Cycloheximide (OD 578 nm) | 0.477 | 0.467 | 0.716 | 0.618 |
Spore forming agar | very good: no spores observed under microscope | |||
Growth on SP medium | − | +/− | + | + |
Enzyme activity | ||||
α-Galactosidase | − | − | + | + |
β-Glucuronidase | − | − | +/− | + |
β-Galactosidase | − | + | + | + |
β-Fucosidase | − | + | + | + |
β-Glucosidase | − | − | + | + |
Chitinase | +/− | + | + | + |
Oxidase | + | + | + | + |
Catalase | + | + | + | + |
Gram-staining | − | − | − | − |