Enhancement of thioredoxin/glutaredoxin-mediated L-cysteine synthesis from S-sulfocysteine increases L-cysteine production in Escherichia coli

Table 1 Bacterial strains and plasmids used

E. coli strain or plasmid	Genotype	Reference or source
Strains
BW25113	wild type	[21]
JW2720	cysC::Km^R	[21]
JW3582	cysE::Km^R	[21]
JW3331	cysG::Km^R	[21]
JW2732	cysH::Km^R	[21]
JW2733	cysI::Km^R	[21]
JW2734	cysJ::Km^R	[21]
JW2407	cysK::Km^R	[21]
JW2414	cysM::Km^R	[21]
AG1		[22]
Plasmids
pDES	pACYC184 with serA (T410 Stop), ydeD and alterd cysE (T167A) genes under the control of the OmpA promoter	[9]
pCA24N	Cm^R	[22]
pCysH	pCA24N, cysH gene on 0.7 kb DNA fragment	[22]
pCysI	pCA24N, cysI gene on 1.7 kb DNA fragment	[22]
pCysJ	pCA24N, cysJ gene on 1.8 kb DNA fragment	[22]
pCysK	pCA24N, cysK gene on 0.9 kb DNA fragment	[22]
pCysM	pCA24N, cysM gene on 0.9 kb DNA fragment	[22]
pGrx1	pCA24N, grxA gene on 0.26 kb DNA fragment	[22]
pGrx2	pCA24N, grxB gene on 0.62 kb DNA fragment	[22]
pGrx3	pCA24N, grxC gene on 0.25 kb DNA fragment	[22]
pGrx4	pCA24N, grxD gene on 0.35 kb DNA fragment	[22]
pTrx1	pCA24N, trxA gene on 0.33 kb DNA fragment	[22]
pTrx2	pCA24N, trxC gene on 0.42 kb DNA fragment	[22]
pNrdH	pCA24N, nrdH gene on 0.25 kb DNA fragment	[22]
pCysK-I	pCA24N, cysK, cysI gene on 2.6 kb DNA fragment	This study
pCysK-J	pCA24N, cysK, cysIJ gene on 2.7 kb DNA fragment	This study
pCysJ-I	pCA24N, cysJ, cysI gene on 3.5 kb DNA fragment	This study
pCysK-I-NrdH	pCA24N, cysK, cysI, nrdH gene on 3.0 kb DNA fragment	This study

ISSN: 1475-2859