Skip to main content
Figure 2 | Microbial Cell Factories

Figure 2

From: Enhancement of thioredoxin/glutaredoxin-mediated L-cysteine synthesis from S-sulfocysteine increases L-cysteine production in Escherichia coli

Figure 2

Reduction of SSC to L-cysteine by Trxs and Grxs in vitro. (a) Reducing activity of SSC by Grxs was measured in a reaction mix containing NADPH, GSH, GSH reductase and SSC by monitoring the decrease of NADPH, when adding 10 pmoles of purified Grxs (Grx1-3) for 3 minutes. (b) Reducing activity of SSC by Trxs was measured in a reaction mix containing NADPH, Trx reductase and SSC by monitoring the decrease in absorbance of NADPH at 340 nm for 3 minutes, when adding 10 pmoles of purified Trxs (Trx1-2, NrdH and Grx4).

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us