Figure 3
From: A novel expression system for production of soluble prion proteins in E. coli
Purification of soluble MoPrP(89-230). A) Purification of MoPrP(89-230) after Ni-NTA. Lane 1: Molecular weight. Lane 2: Lysate (soluble fraction). Lane 3: Flow through. Lane 4 to 9: Elution peak. B) Size exclusion chromatogram (Superdex75 HR10/30). C) SDS/PAGE of pooled fractions from the highest elution peak (from 13 to 16 mL elution volume). Lane 1: Molecular weight. Lane 2: pooled fractions contain purified PrP.