Figure 2
From: A novel expression system for production of soluble prion proteins in E. coli
Production of MoPrP(89-230) in E. coli. A) Growth curve of E. coli cells, showing the expression of MoPrP(89-230): absence of QSOX and co-expressed with QSOX in black and in red respectively. B) SDS-PAGE of total cell extract. Lane 1: (+) QSOX before induction, lane 2: (-) QSOX before induction, 3: (+) QSOX 1 h after induction, lane 4: (-) QSOX 1 h after induction, lane 5: (+) QSOX 2 h after induction, lane 6: (-) QSOX 2 h after induction, lane 7: (+) QSOX 4 h after induction, lane 8: (-) QSOX 4 h after induction, lane 9: (+) QSOX 8 h after induction, lane 10: (-) QSOX 8 h after induction, lane 11: (+) QSOX 16 h after induction, lane 12: (-) QSOX 16 h after induction and lane 13: MW. C) Immunoblot showing the expression level of total MoPrP(89-230) over time. D) Quantification of the total production of MoPrP(89-230) expressed over 16 h. Intensities from the western blot signal were quantified using the LabImage software, in presence of QSOX and in absence of QSOX in red and green respectively. E) Immunoblot showing the expression level of soluble MoPrP(89-230) over time (same time points as in B). F) Quantification of the production of soluble MoPrP(89-230) expressed over 16 h, in presence of QSOX and in absence of QSOX in red and green respectively.