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Table 4 Amino acid composition of  P. pastoris 

From: Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures

 

80% Glucose 20% Methanol

100% Glucose

% mol/mol

X-33

ROL 1-copy

ROL 2-copy

X-33*

 S. cerevisiae **

Arg

8.15

7.67

6.45

7.04

3.86

Asp

9.25

9.48

10.05

8.78

9.28

Thr

6.15

6.06

6.46

5.88

5.57

Ser

6.34

6.32

6.82

6.26

5.33

Glu

15.76

15.44

13.23

17.81

15.48

Pro

4.2

4.0

3.92

3.83

4.22

Gly

7.80

7.53

7.86

6.86

8.89

Ala

11.14

9.41

9.36

10.40

9.77

Val

6.81

6.67

6.54

5.88

7.33

Cys

0.17

0.19

0.13

0.15

0.14

Met

0.78

0.75

0.62

0.79

1.14

Ile

4.49

4.49

4.69

4.64

5.89

Leu

7.50

7.45

8.34

6.96

8.01

Tyr

2.28

2.19

2.51

2.16

1.96

Phe

3.44

3.31

3.61

3.20

3.76

Orn

0.68

0.32

0.26

1.04

0.24

Lys

6.77

6.77

7.21

6.41

6.57

His

2.10

1.94

1.95

1.89

1.93

Trp

1.00

1.00

1.00

1.40

1.96

  1. * Data taken from [26]; ** Data taken from [27].
  2. Amino acid composition of the whole cell extract for all the P. pastoris strains tested. Amino acid composition measured for the three different ROL gene dosages. Data given as percentage of each amino acid in the total pool of amino acids. The standard deviation of the analytical method was 3~5%.
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Microbial Cell Factories

ISSN: 1475-2859

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