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Table 2 Biomass C-molecular composition for  P. pastoris 

From: Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures

A

Strain

Carbon source

C-mol Biomass formula

C:N Ratio

H:O Ratio

γ

X-33 control

80% Glucose 20% Methanol

CH1.687N0.17 O0.635S0.002

5.9

2.7

3.9

ROL 1-copy

80% Glucose 20% Methanol

CH1.749N0.141 O0.679S0.002

7.1

2.6

4.0

ROL 2-copy

80% Glucose 20% Methanol

CH1.702N0.14 O0.643S0.002

7.1

2,6

4.0

X-33 control*

100% Glucose

CH1.761N0.143 O0.636S0.0018

7.0

2.8

4.1

S. cerevisiae**

100% Glucose

CH1.748N0.148 O0.596S0.0018

6.8

2.9

4.2

  1. *Data taken from [26] ; ** Data taken from [27].
  2. Biomass C-molecular formula for P. pastoris growing on glucose:methanol mixture (80:20) as a carbon source in chemostat cultures at a D = 0.09 h−1, expressed as C-molecular formula. C:N, carbon:nitrogen ratio; H:O, hydrogen:oxygen ratio. γ, reduction degree of the biomass.
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Microbial Cell Factories

ISSN: 1475-2859

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