Figure 5

Fluorescence recovery in the presence of small chemical compounds. Aβ42wt-GFP IBs were denatured in 8 M Gu·HCl and diluted 100-fold in PBS in the absence or presence of 25 μM Cu⁺² (A) and Zn⁺² (B) and in the presence of the following small chemical compounds at 25 μM final concentration: azure C (C1), basic blue 41 (C2), meclocycline sulfosalicylate (C3), hemin chloride (C4), o-Vanillin (C5), quercetin (C6), congo red (C7), thioflavin –T (C8), apigenin (C9), nordihydroguaiaretic acid (C10), myricetin (C11) and curcumin (C12). (C) Quenching of native untagged GFP fluorescence by 25 μM o-Vanillin. (D) Comparative effect of the absence or presence of 25 μM o-Vanillin and/or Zn⁺² on GFP fluorescence recovery upon dilution of denatured Aβ42wt-GFP IBs. (E) GFP fluorescence recovery kinetics upon dilution of denatured Aβ42wt-GFP IBs in PBS (black solid circles) and PBS with 25 μM Cu⁺² (red) or Zn⁺² (blue) ions in the absence (solid circles) or presence (empty circles) of 25 μM o-Vanillin.