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Table 1 Copy number of dpgA and groEL-groES genes and D-PhgAT activity of different P. pastoris clones

From: Effective enhancement of Pseudomonas stutzeri D-phenylglycine aminotransferase functional expression in Pichia pastoris by co-expressing Escherichia coli GroEL-GroES

Strain name

Host strain

Integrated plasmid

Integration site

Copy number

Specific D-PhgAT activity [U·mg-1]a

Volumetric D-PhgAT activity

[U·L-1]b

    

dpgA

groEL-groES

  

KM_AT3

KM71

pPIC3.5K_

HIS4

3

0

0.0054

2.9

  

D-PhgAT

     

KM_AT1_ELS1

KM71

pAO_

HIS4

1

1

12.6

7531

  

D-PhgAT_GAP _ ELS

     

KM_AT3_ELS3

KM71_D-PhgAT

pPICZ_26S_GAP_ELS

5′AOX1

3

3

23.3

14558

KM_AT3_ELS4

  

5′AOX1

3

4

45.3

34809

KM_AT3_ELS2

  

26S rDNA

3

2

14.6

11027

KM_AT3_ELS10

  

26S rDNA

3

10

60.1

41730

  1. Cells were cultured at 30°C.
  2. a Specific activity was calculated as unit of D-PhgAT activity per mg of soluble cellular protein.
  3. b Volumetric activity was calculated as unit of D-PhgAT activity obtained per liter of P. pastoris culture.
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Microbial Cell Factories

ISSN: 1475-2859

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