Comparison of D-PhgAT expressions from P. pastoris KM71 strains carrying different copy numbers of dpgA and groEL-groES genes. SDS-PAGE analysis of soluble (a) and insoluble (b) fractions from 6 recombinant strains. Lane M, molecular mass markers; Lane 1, strain KM_AT3; Lane 2; strain KM_AT1_ELS1; Lane 3–6; strains KM_AT3_ELS3, KM_AT3_ELS4, KM_AT3_ELS2, KM_AT3_ELS10, respectively. Please see Table 1 for details of each strain. The protein bands corresponding to D-PhgAT (45 kDa) and GroEL (60 kDa) are arrow indicated.