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Figure 7 | Microbial Cell Factories

Figure 7

From: Effective enhancement of Pseudomonas stutzeri D-phenylglycine aminotransferase functional expression in Pichia pastoris by co-expressing Escherichia coli GroEL-GroES

Figure 7

Comparison of D-PhgAT expressions from P. pastoris KM71 strains carrying different copy numbers of dpgA and groEL-groES genes. SDS-PAGE analysis of soluble (a) and insoluble (b) fractions from 6 recombinant strains. Lane M, molecular mass markers; Lane 1, strain KM_AT3; Lane 2; strain KM_AT1_ELS1; Lane 3–6; strains KM_AT3_ELS3, KM_AT3_ELS4, KM_AT3_ELS2, KM_AT3_ELS10, respectively. Please see Table 1 for details of each strain. The protein bands corresponding to D-PhgAT (45 kDa) and GroEL (60 kDa) are arrow indicated.

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