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Figure 6 | Microbial Cell Factories

Figure 6

From: Effective enhancement of Pseudomonas stutzeri D-phenylglycine aminotransferase functional expression in Pichia pastoris by co-expressing Escherichia coli GroEL-GroES

Figure 6

SDS-PAGE analysis of soluble (S) and insoluble (I) protein fractions from P. pastoris KM71 expressing D-PhgAT alone and with GroEL-GroES co-expression. Lane M, molecular mass markers; pAO, clone with blank pAO815; pAO_AT, clone expressing dpgA under P AOX1 ; pAO_AT_AOX_ELS, clone expressing P AOX1 dpgA with co-expressing P AOX1 groEL and P AOX1 groES; pAO_AT_GAP_ELS, clone expressing P AOX1 dpgA with co-expressing P GAP groEL and P GAP groES. The protein bands corresponding to D-PhgAT (45‚ÄČkDa) and GroEL (60‚ÄČkDa) are arrow indicated.

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