Figure 4

Adh1p aggregation resulting from oxidative carbonylation. Anti-Adh1p western blot (upper panel) for the T73 and TTRX2 strains from dehydrated cells and cells grown in YPGF medium for 5 h (A) or from fresh YPGF growing cells, treated or not treated with carbonylation inductor glyoxal (GO) (B). The bottom panel shows the Coomassie-stained membrane used as a control of protein amount. (C) Anti-DNP western blot of Adh1p immunoprecipitated samples: ¹ T73 and ² T73+5 mM glyoxal. Marked bands correspond to the dimer (74 KDa) and tetramer (148 KDa) forms of Adh1p.