Table 5 Strains and plasmids used in this study

E. coli W1485

F ⁺ rpoS396(Am) rph-1

Lab stocked

E. coli BL21 (DE3)

F' ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1ind1 sam7 nin5])

Novagen

E. coli TOP10

F' mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZ ΔM15 ΔlacΧ74 recA1 araD139Δ(ara-leu) 7697 galU galK rpsL (Strr) endA1 nupG

Invitrogen

Z0001

BL21 (DE3): Δlpp

This study

Z0003

W1485: ΔldhA, ΔptsG::cat, ΔpflB::kan

This study

Z1000

BL21 (DE3): pTrc99z

This study

Z1EX0

BL21 (DE3): pA-E_mX_n

This study

Z1371

Z0001: pA-E₃X₇

This study

Z2370

BL21 (DE3): pA-E₃X₇D

This study

Z3000

BL21 (DE3): pTrc15a

This study

Z3370

BL21 (DE3): pB-E₃X₇

This study

Z3371

Z0001: pB-E₃X₇

This study

Z4370

BL21 (DE3): pB-E₃X₇D

This study

Z5370

BL21 (DE3): pB-E₃X₇AD

This study

Z6373

Z0003: pB-E₃X₇AYD

This study

Plasmid

Description

Source

pUC57-E_m

pMB1 ori, P _lac : endoxylanase gene (E₁, E₂ or E₃ gene), Amp^r

Genscript

pUC57-X_n

pMB1 ori, P _lac : xylosidase gene (X₄, X₅, X₆ or X₇ gene), Amp^r

Genscript

pUC57-E_mX_n series

pMB1 ori, P _lac :osmY-endoxylanase E_m, osmY-xylosidase X_n, Amp^r

This study

pTrc99a

pBR322 ori, Amp^r

Invitrogen

pTrc99z

pBR322 ori, Amp^r

This study

pA-E_mX_n series

pBR322 ori, P _trc :osmY-endoxylanase E_m, osmY-xylosidase X_n, Amp^r

This study

pA-E₃X₇D

pBR322 ori, P _trc : osmY-xynC-A, osmY-xyloA; PdsbA: dsbA, Amp^r

This study

pACYC184

p15a ori, Cat^r Tet^r

NEB

pTrc15a

p15a ori, Amp^r

This study

pB-E₃X₇

p15a ori, P _trc : osmY-xynC-A, osmY-xyloA, Amp^r

This study

pB-E₃X₇D

p15a ori, P _trc : osmY-xynC-A, osmY-xyloA; PdsbA: dsbA, Amp^r

This study

pB-E₃X₇OD

p15a ori, P _trc : osmY-xynC-A, osmY-xyloA, osmY; PdsbA: dsbA, Amp^r

This study

pB-E₃X₇AD

p15a ori, P _trc : osmY-xynC-A, osmY-xyloA, osmY-abf2; PdsbA: dsbA, Amp^r

This study

pB-E₃X₇AYD

p15a ori, P _trc : osmY-xynC-A, osmY-xyloA, osmY-abf2; P _trc : pyc; PdsbA: dsbA, Amp^r

This study