Table 3 Primers used in this study
Primers | Nucleotide sequence |
|---|---|
trpR-F | 5'-GGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTGTAGGCTGGAGCTGCTTC-3' |
trpR-R | 5'-ACGGGTATTTGTAGGACGGATAAGGCGTTCACGCCGCATATGGGAATTAGCCATGGTCC-3' |
tnaA-F | 5'-CAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGTGTAGGCTGGAGCTGCTTC-3' |
tnaA-R | 5'-TGTTTACCGGTTTTCGGATCGCGGCCTAACAGGAAAGAGATGGGAATTAGCCATGGTCC-3' |
ptsG-F | 5'-ACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTGTGTAGGCTGGAGCTGCTTC-3' |
ptsG-R | 5'-AGCCATCTGGCTGCCTTAGTCTCCCCAACGTCTTACGGAATGGGAATTAGCCATGGTCC-3' |
kan-F | 5'-ATAGGATCC GTGTAGGCTGGAGCTGCTTC-3' |
kan-R | 5'-GGGGAATTC ATGGGAATTAGCCATGGTCC-3' |
Mtac-F | 5'-GGGGAATTC TTGACAATTAATCATCGGCTCGTATA-3' |
Mtac-R | 5'-GGGGTCGAC ATGCATCTAGTATTTCTCCTCTTTAATGGAT-3' |
trp-F | 5'-GTGCAGGTCGTAAATCACTGCATAATTCGTGTCGCTCAAGTGTAGGCTGGAGCTGCTTC-3' |
trp-R | 5'-GCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATATGCATCTAGTATTTCTCCT-3' |
trp test-F | 5'-GCCTTACCGCCAGAATGATGAATGA-3' |
trp test-R | 5'-GCAGCACACGGCAGTTTGGTGATTG-3' |
trpRtest-F | 5'-GTGCTGGCTTATGACGCTTACTACCGCTAT-3' |
trpRtest-R | 5'-CGCTGAGTCCGTTTCATAATGCCGTGTA-3' |
tnaAtest-F | 5'-TATCAATACACCATTCCGACTCACC-3' |
tnaAtest-R | 5'-GTGAAGTGACGCAATACTTTCGGTT-3' |
ptsGtest-F | 5'-CCTGTACACGGCGAGGCTCT-3' |
ptsGtest-R | 5'-AATAACACCTGTAAAAAAGGCAGCC-3' |
trpEFR -F | 5'-GCCAAGCTT AAGGAGATATAATGCAAACACAAAAACCGAC-3' |
trpEFR -R | 5'-ATTAACTGCAG TCAGAAAGTCTCCTGTGCATGATGCG-3' |
aroGFR -F | 5'-GGGGCCTGCAG AAGGAGATATAATGAATTATCAGAACGACG-3' |
aroGFR -R | 5'-TTATTGGATCC TTACCCGCGACGCGCTTTTACTGCATT-3' |
tktA-F | 5'-GGCGGATCC AAGGAGATATAATGTCCTCACGTAAAGAG-3' |
tktA-R | 5'-CGTGAGCTC TTACAGCAGTTCTTTTGCTTTCGCAACAACG-3' |
Primers for RT-PCR | |
trpERT-F | 5'-CACAATCCAGGCACTTTCCG-3' |
trpERT-R | 5'-GGCGTCTTCATCCAGCAGTG-3' |
trpDRT-F | 5'-GTGCTGATGCTTTCTCCTGG-3' |
trpDRT-R | 5'-CCTGATGTCCGAGGCAAATG-3' |
trpCRT-F | 5'-CAGACAAGGCGATTTGGGTA-3' |
trpCRT-R | 5'-TTGACGGCGACGCTTTCTTG-3' |
trpBRT-F | 5'-GGCAGGCGTTGCTGGCGAAG-3' |
trpBRT-R | 5'-GTTAGGCGACTGGCGTTCAA-3' |
trpART-F | 5'-TCTGTTTGCCCAGTTGAAGG-3' |
trpART-R | 5'-GGGATACCTAACTCCAGCG-3' |
aroGRT-F | 5'-TGGGCTGGAAAGGGCTGATT-3' |
aroGRT-R | 5'-GAGAAACTCACCTGCCGCTG-3' |
gapART-F | 5'-AACTGAATGGCAAACTGACTGGTA-3' |
gapART-R | 5'-TTTCATTTCGCCTTCAGCAGC-3' |