Table 2 Strains and plasmids used in this study
Strains | Genotype | Reference |
|---|---|---|
W3110 | F-, λ-, rph-1, IN (rrnD, rrnE) | Lab stock |
DH5α | F-, endA1, hsdR17 (r K -, m K +), supE44, thi-l, λ-, recA1, gyrA96, ΔlacU169 (Φ80lacZ ΔM15) | Lab stock |
GPT100 | W3110 ΔtrpR::FRT, ΔtnaA::FRT, ΔptsG::FRT | This study |
GPT101 | GPT100 with tryptophan attenuator deletion and trp promoter swapping by 5CPtacs promoter cluster | This study |
GPT1001 | GPT100 containing pTAT | This study |
GPT1002 | GPT101 containing pTAT | This study |
Plasmids | Genotype | Reference |
pBluescript SK- | ApR | Lab stock |
pCL1920 | SpcR | [33] |
pTAT | pCL1920 containing aroGFR, trpEFR, and tktA | This study |
pKD4 | bla, FRT-cat-FRT | [34] |
pKD3 | bla, FRT-kan-FRT | [34] |
pKD46 | bla, helper plasmid | [34] |
pCP20 | bla and cat, helper plasmid | [35] |
p5TG | pCL1920 containing 5CPtacs promoter cluster and gfp | [22] |
pKMT | pBluescript SK-, containing kan and 5CPtacs promoter cluster | This study |