Figure 1

The strategies for constructing the L-tryptophan producing strain GPT1002. The shaded boxes represent genetic modification, and the gray bars indicate the genes that were deleted. Dotted lines indicate feedback inhibition. The black X indicates that the inhibition is removed. The thick black arrows indicate the increased flux or activity by directly overexpressing the corresponding genes in plasmids. Glc glucose, G6P glucose-6-phosphate, E4P erythrose-4-phosphate, PEP phosphoenolpyruvate, DAHP 3-deoxy-D-arabino-heptulosonate, CHA chorismate, ANTA anthranilate, L-Phe L-phenylalanine, L-Tyr L-tyrosine, L-Trp L-tryptophan, tktA transketolase, aroG 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (phenylalanine repressible), trpE component I of anthranilate synthase, trpR trp operon repressor, tnaA tryptophanase, ptsG glucose-specific PTS enzyme IIBC components, manXYZ mannose-specific PTS enzyme IIABCD components, galP D-galactose transporter, PP pathway pentose phosphate pathway.