Figure 4
Repression of the auto-inducuble system. Comparison of efficiency of repression of the auto-inducible system by transcriptional interference on luxR. pMKSal-gpf /MM294.1, pMKSal-gpf /BL21DE3 and pMKSal/MM294.1 were grown in LB at 37°C with shaking. For each plasmid/strain combination, 3 replicates were incubated for each of the 4 concentrations of IPTG: 0, 0.5, 1 and 5 mM. After 16 h of growth, GFP expression was determined. pMKSal-gpf /MM294.1 and pMKSal/MM294.1 combinations were used as positive and negative controls. Error bars show the standard deviation of experiments. RFU/OD590, Relative Fluorescence Unit per OD590 unit.