Figure 3

SDS-PAGE of BL21 cell lysates harboring the recombinant plasmids. (A). pELipAB-LipB1^a (1-8) and pELipB1^k (9-10). LB medium without addititive (1-2), with 0.5% (v/v) of Neptune oil (5-6), 0.5% (w/v) of gum Arabic (3-4), 0.5% (w/v) of gum Arabic + 0.5% (v/v) of Neptune oil (7-8). Odd and even lanes indicated induction by IPTG for 0 and 4 hours, respectively. (B). pELipB3^k (1-2) and pELipAB-LipB3^a (3-10). LB medium without additive (3-4), with 0.5% (w/v) of gum Arabic (5-6), 0.5% (v/v) of Neptune oil (7-8), 0.5% (w/v) of gum Arabic + 0.5% (v/v) of Neptune oil (9-10). Odd and even lanes indicated induction by IPTG for 0 and 4 hours, respectively. (C). pELipAB^a (1); pELipAB-LipB3^a (2-4), pELipAB-LipB1^a (5-7). LB medium without additive (1, 2, 5), with 0.5% of Tween 80 (3, 6), and 0.5% of Triton X-100 (4, 7) after induction by IPTG for 4 hours.