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Table 1 Components of auto-induction media.

From: Recombinant production of the therapeutic peptide lunasin

Medium components

Growth Medium

 
 

TB

4ZY

8ZY

8ZY + succinate

Tryptone (g/L) [Z]

12

40

80

80

Yeast extract (g/L) [Y]

24

20

40

40

NPCS (mM)

    

   Na2HPO4

25

25

25

25

   KH2PO4

25

25

25

25

   NH4CI

50

50

50

50

   Na2SO4

5

5

5

5

MgSO4(mM)

2

2

2

2

Trace metals

0.2×

0.2×

0.2×

0.2×

5052 (%)

    

   Glycerol

2

2

2

2

   Glucose

0.2

0.2

0.2

0.2

   Lactose

0.8

0.8

0.8

0.8

Succinate (mM)

-

-

-

25

  1. Adapted from [12]
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Microbial Cell Factories

ISSN: 1475-2859

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