Figure 4

E. coli cell morphology expressing CBD-lunasin. Light microscopy image of Gram stained E. coli BL21* (DE3) expressing (A) CBD-lunasin fusion protein and (B) CBD alone. Note the absence of the elongated non-septate forms of E. coli BL21*(DE3) in cells expressing CBD alone. (C) TEM image of the elongated, non-septate morphology of subsets of E. coli BL21*(DE3) expressing the CBD-lunasin fusion protein. Two distinct regions can be identified which are indicated by white arrows. It is likely that the darker regions represent the aggregation of fusion protein into insoluble inclusion bodies. In contrast the lighter regions are likely to represent the soluble fusion protein.