Figure 3
From: Recombinant production of the therapeutic peptide lunasin
Expression of CBD-lunasin constructs and protein recovery. (A) SDS-PAGE analysis of soluble and insoluble fractions of E. coli BL21*(DE3) carrying pET28-CBD-lunasin grown on 8ZY auto-induction media supplemented with 4 × 5052 and succinate at 25°C. (B) Time-course of cell growth as OD600 in various auto-induction media at 25°C (C) SDS-PAGE analysis of the CBD-lunasin fusion protein purified using cellulose phosphate batch binding, cleavage by TEV protease and either a Microcon® concentrator column or Ni-NTA agarose step. (D) CBD-lunasin fusion protein yield over time-course in (B).