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Figure 8 | Microbial Cell Factories

Figure 8

From: L-Rhamnose induction of Aspergillus nidulans α-L-rhamnosidase genes is glucose repressed via a CreA-independent mechanism acting at the level of inducer uptake

Figure 8

Co-utilization of rhamnose and ethanol. Time-course measurement of the amount of rhamnose or ethanol remaining in the cultures during cultivation of the A. nidulans creAd30 strain in MM containing either 1% w/v rhamnose (R) (Panel A) or a mixture of 1% w/v rhamnose and an equimolar amount of ethanol (E) (Panel B). Symbols: (•) concentration of rhamnose, (Δ) concentration of ethanol. Data points are represented as the mean and standard deviation of three independent experiments. α-L-rhamnosidase activity was tested by spotting 10 μl of the same cultures on agar plates containing MUR. (Panel C) α-L-rhamnosidase activity after transfer to the rhamnose media at the times indicated. (Panel D) A sector of a Petri dish showing the lack of α-L-rhamnosidase activity in the mixture cultures.

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