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Figure 2 | Microbial Cell Factories

Figure 2

From: Characterization of a thermostable β-glucosidase from Aspergillus fumigatus Z5, and its functional expression in Pichia pastoris X33

Figure 2

Alignment of sequences deduced from A. fumigatus nBgl3 with other cellulases using BioEdit. The sequences are numbered: 1 (Aspergillus fumigatus Z5 nBgl3), 2 (Aspergillus clavatus NRRL 1 putative β-glucosidase: XP_001269582.1), 3 (Aspergillus flavus NRRL3357 putative β-glucosidase: XP_002383240.1), 4 (Aspergillus niger β-glucosidase: CBA02054.1), 5 (Aspergillus avenaceus β-glucosidase: AAX39011.1), 6 (Paracoccidioides brasiliensis Pb01 β-glucosidase: XP_002795910.1), 7 (Arthroderma benhamiae CBS 112371 putative β-glucosidase: EFE35611.1), and 8 (consensus); the predicted signal peptide sequence is shown in the box. The conserved region used for the degenerate PCR primer is marked by a straight line, the active site is marked by a broken line, and the putative nucleophile in the catalytic center is marked by an inverse triangle.

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