Table 4 Primers used in this study for gene cloning and plasmid construction
From: Reconstruction of cytosolic fumaric acid biosynthetic pathways in Saccharomyces cerevisiae
Oligonucleotides | Sequences, 5'-3' | Usage |
|---|---|---|
F-RoFUM1 | ATGTTGCGAGCTTCTGCTACC | Cloning of RoFUM1 |
R-RoFUM1 | TTAATCCTTGGCAGAGATCATATCTT | |
F-RoMDH | ATGTTTGCCGCCTCTCGTG | Cloning of RoMDH |
R-RoMDH | TTATTGAACAAAGCTGTTACCCTTG | |
BamHI-F(RoMDH) | CGGGATCC ATGTTTGCCGCCTCTCGTG | Construction of pY26TEF-GPD-RoMDH |
HindIII-R(RoMDH) | CCCAAGCTT TTATTGAACAAAGCTGTTACCCTTG | |
BamHI-F(MDH3ΔSKL) | CGGGATCC ATGGTCAAAGTCGCAATTCTTG | Construction of pY26TEF-GPD- MDH3ΔSKL |
HindIII-R(MDH3ΔSKL) | CCCAAGCTT TCAAGAGTCTAGGATGAAACTCTTGCCT | |
NotI-F(RoFUM1) | ATAAGAATGCGGCCGC ATGTTGCGAGCTTCTGCTACC | Construction of pY26TEF- RoFUM1- GPD- RoMDH |
BglII-R(RoFUM1) | GAAGATCT TTAATCCTTGGCAGAGATCATATCTT | |
PstI -F(PYC2) | AACTGCAG ATGAGCAGTAGCAAGAAATTGGC | Construction of pRS305TEF1-PYC2 |
SalI -R(PYC2) | ACGCGTCGAC TTACTTTTTTTGGGATGGGGGT |