Figure 5
From: IcsA autotransporter passenger promotes increased fusion protein expression on the cell surface
Detection of IcsA and IcsA β fusion proteins on the surface of UT5600 strains. Immunofluorescence microscopy showing surface presentation of pBAD33 (empty vector) (MLRM3), IcsA (MLRM4), IcsA-Bla (MLRM28), IcsAβ-Bla (MLRM20), IcsA-MalE (MLRM39) and IcsAβ-MalE (MLRM37) with (A) anti-IcsA; (B) anti-Bla; (C) anti-MBP. Phase contrast microscopy (upper panels) and fluorescence microscopy (lower panels) were performed. Specific proteins were detected with their respective antibodies and were detected by FITC-labelled anti-rabbit immunoglobulin serum. The relative percentage of FITC-positive cells are indicated (n = 100)