Figure 4
From: IcsA autotransporter passenger promotes increased fusion protein expression on the cell surface
Detection of IcsA and IcsA β fusion proteins in the OM. UT5600 strains were grown to mid-log phase, induced with 0.2% (w/v) arabinose and were fractionated into soluble fraction (S), whole membrane (WM), inner membrane (IM) and outer membrane (OM). Equivalent amounts of protein extracts from each fraction were loaded and analysed by Western immunoblotting. Immunobloting of fractions from (A) MLRM28 (pMLRM1::bla) and MLRM20 (pMLRM2::bla) with anti-Bla; (B) MLRM28 (pMLRM1::bla) with anti-IcsA; (C) MLRM39 (pMLRM1::malE) with anti-MBP and (D) anti-IcsA. The apparent molecular sizes of the proteins are indicated.